Carotenoid production of Botryococcus braunii CCAP 807/2 under different growth conditions

Botryococcus braunii CCAP 807/2 has been studied intensively for biofuel production due to its high hydrocarbon content. This strain is also capable of producing value carotenoids. The aim the study was analyse carotenoid B.braunii under different growing conditions, first, by using media and light intensities in indoors, next, examine composition between green, intermediately pigmented red B. grown indoors outdoors. alga cultured two (100 500 μmol photons m−2 s−1) three media: a control with complete modified CHU 13 medium, without N CHU13 + 2Fe. All cultures were at 25 °C 12:12 h light:dark cycle mixed magnetic stirrers. For determination stages, cells collected from indoor outdoor analysed their HPLC. intensity reached highest biomass yield 0.6 g L−1 on day 16, whereas counterparts low took 30 days reach same yield. increased up twofold 2 compared ones light. accumulates lutein, canthaxanthin astaxanthin β,β-carotene as main Whilst lutein major carotenoids green/intermediate cells, predominant culture, respectively. suggests that potential candidate and/or astaxanthin. It amount when optimum conditions sub-optimum